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Research on syntactic ambiguity resolution in language comprehension has shown that subjects' processing decisions are influenced by a variety of heterogeneous factors such as e.g., syntactic complexity, semantic fit and the discourse frequency of the competing structures. The present paper investigates a further potentially relevant factor in such processes: effects of syntagmatic lexical chunking (or matching to a complex memorized prefab) whose occurrence would be predicted from usage-based assumptions about linguistic categorisation. Focusing on the widely studied so-called DO/SC-ambiguity in which a post-verbal NP is syntactically ambiguous between a direct object and the subject of an embedded clause, potentially biasing collocational chunks of the relevant type are identified in a number of corpus-linguistic pretests and then investigated in a self-paced reading experiment. The results show a significant increase in processing difficulty from a collocationally neutral over a lexically biasing to a strongly biasing condition. This suggests that syntagmatically complex and partially schematic templates of the kind envisioned in usage-based Construction Grammar may impinge on speakers' online processing decisions during sentence comprehension.